Dr. Shi-Bing Su
Shanghai University of Traditional Chinese Medicine, China
Title: Discovery of potential biomarkers from chronic hepatitis B to cirrhosis by cytokine profiling analysis
Biography
Biography: Dr. Shi-Bing Su
Abstract
Background: Liver cirrhosis is a critical state in the natural course of hepatocellular carcinoma (HCC). Chronic hepatitis B (CHB) is a major cause of liver cirrhosis in China. To find biomarkers for the diagnosis of CHB caused cirrhosis (HBC), we examined the cytokines profiling of CHB and HBC. Methods: Serum samples of 15 health controls (HC) and 15 CHB patients and 15 HBC patients were collected to detect the profiles of 48 cytokines by multiplex biometric ELISA-based immunoassay. Partial least squares discriminant analysis (PLS-DA) was used to analyze the significant cytokines, and they were validated using independent cohort of 60 CHB patients, 60 HBC patients and 35 HC samples. Results: There were 22 differential expressed cytokines of CHB and HBC. Three differential expression cytokines including interleukin (IL)-9, IL-2 receptor subunit alpha (IL2Rα) and Granulocyte-macrophage colony-stimulating factor (GM-CSF) were found by PLS-DA, and their significant changes of serum levels were further validated. The Receiver-operator characteristic (ROC) analysis demonstrated that three cytokines and their logistic regression panel potentially to be the potential biomarkers for CHB and HBC differentiation (P<0.001, AUC=0.876). Furthermore, a functional pathway analysis showed that differential regulation of cytokine production in macrophages, T Helper Cells and intestinal epithelial cells by IL-17A and IL-17F were enriched in the 22 differentially expressed cytokines. They were also enriched in pathway of hepatic fibrosis / hepatic stellate cell activation. Conclusions: There were particular cytokines profiles of CHB and HBC. Besides, IL-9, IL2Rα and GM-CSF may be involved in the differentiation of CHB and HBC.These findings may give further insight into the pathobiology of HBC.
